Formation of 2D & 3D Crysalins
With the aim of facilitating the structural characterization of biological macromolecules, Crysalin has produced a novel type of crystalline material by means of the genetic fusion of oligomeric proteins of appropriate symmetry (outlined in the original crysalin patent, GB2393959).
Crysalin lattices are formed from a combination of high symmetry protein oligomers belonging to Tetrahedral (T), Octahedral (O), Dihedral (Dn) and Cyclic (Cn) point groups. They employ the intrinsic symmetry of these oligomeric proteins to generate lattices via genetic fusion to other proteins with appropriately matched symmetry and coordination properties. Each protein fusion is termed a crysalin â€œprotomerâ€.
We are able to routinely produce 1D, 2D and 3D Crysalins.
These engineered lattices have properties that will make them suitable as frameworks for the spatially oriented attachment of guest macromolecules. This provides a route, via x-ray diffraction or EM, to rapid and automated structure determination of macromolecules and their complexes with macromolecular partners and with small molecule co-factors, substrates and drugs.
Crysalin assembly principles